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The American healthcare researcher Fred Bang documented in 1956 that gram-unfavorable bacteria, even though killed, will cause the blood of your horseshoe crab to turn into a gel, a sort of semi-reliable mass. It absolutely was later on recognized the animal's blood cells, cellular cells known as amebocytes, incorporate granules using a clotting is

The reference electrode typically includes a platinum wire which has contact with a silver/silver chloride combination, that's immersed in the potassium chloride Answer. There exists a ceramic plug, which serves like a Get in touch with to the test Resolution, giving reduced resistance although blocking mixing of The 2 methods.[13]:?76–ninety one